1205 INDUSTRIAL HIGHWAY ~ PO BOX 514 ~ SOUTHAMPTON,
PA ~ 215 355-3900
Microbial Challenge Study of the MICROCON® MAP
800 M Air Purifier
 The following study was performed
by the Q. C. pharmaceutical microbiology
department from the dates Monday, September 8th
until Monday, September 15th, 1997 for Biological
Controls, Eatontown, NJ.
Procedure
The
MICROCON® MAP 800 M air purifier was challenged
with a known concentration of the following
five
microorganisms;
1. Aspergillis niger ATCC # 16404
2. Candida albicans ATCC # 10231
3. Pseudomonas aeruginosa ATCC # 9027
4. Staphylococcus aureus ATCC # 6538
5. Serratia marcesens ATCC # 14756
The unit was
allowed to run with the UV lights on for a minimum
of two hours prior to challenge in order for it
to clean itself. Ten membrane filters (47 mm, 0.45um)
were then placed on the unit, five on the iar intake
screen and five post HEPA filters just below the
series of UV lights. A known concentration of bacteria
or fungi was then sprayed onto the intake screen
with the unit in full operation with and without
the UV light engaged. The unit was allowed to continue
running for a period od fifteen minutes after the
spraying at which time the filters were especially
removed and placed onto petri dishes containing
either Tryptiucase Soy Agar (bacteria) or Sabouraud
Dextrose
Agar (fungi). Bacteria plates were incubated at
35 +/- 2° C. for 48 hours while fungi plates were
incubated at 25° C. for up to five days. In addition, after removal of the
membrane filters, the unit was allowed to run with
the UV light on for a period of eighteen hours
at which time a series of six swabs were taken
in different locations (three from the intake screen
area and three from the post HEPA filter area.) One organism was tested per
day. The results are recorded on the following pages. Please note that TNTC
is an abbreviation for “too numerous
to count.”
1205 INDUSTRIAL HIGHWAY ~ PO BOX
514 ~ SOUTHAMPTON, PA ~ 215 355-3900
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